Solar powered biohydrogen production requires specific localization of the hydrogenase† †Electronic supplementary information (ESI) available: Supplementary Fig. 1–12 and supplementary Table 1. See DOI: 10.1039/c4ee02502d Click here for additional data file.

Supplementary Figure 1. Chromosomal map of the hox operon in Synechocystis sp. PCC 6803. Schematic showing integration of the eGFP cassette at the C-terminus of hoxF. OriT(RK2) in the disruption cassette stands for origin of transfer and allows for conjugation. The cassette also includes a 19bp and 20bp primer site called P1 and P2 respectively. These are identical in all disruption cassettes. The resistance genes are also flanked by FRT sites (FLP-mediated recognition targets) which allow FLP-mediated excision of the disruption cassette. The size of the cassette is~ 2100bp. The primers hoxFFS and hoxFRS were used to check for complete segregation of the cassette.